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reducing or nonreducing loading buffer (Beyotime)

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Beyotime reducing or nonreducing loading buffer
Reducing Or Nonreducing Loading Buffer, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nonreducing-loading+buffer/non+reducing+loading+buffer/pmc09433816-52-4-6
Average 90 stars, based on 1 article reviews

reducing or nonreducing loading buffer - by Bioz Stars, 2026-09

90/100 stars

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Article Title: Development of a variant of dinutuximab with enhanced antitumor efficacy and reduced induction of neuropathic pain
Article Snippet: A reducing or nonreducing loading buffer (Beyotime, Shanghai, China) was added to 3 μg protein, and the mixture was heated at 100 °C for 10 min in order to obtain the heat‐denatured protein.

Concentration Assay:

Article Title: Cytokines in the Immune Microenvironment Change the Glycosylation of IgG by Regulating Intracellular Glycosyltransferases.
Article Snippet: .. Samples of F(ab’)2 and Fc fragments were diluted to a concentration of 0.5 mg/mL in nonreducing loading buffer (Beyotime, China) containing 5% SDS. .. Furthermore, the samples were denatured by heating at 70°C for 5 min. A total of 2 mg IgG sample per well was loaded for nonreducing SDSPAGE with 4-12% ExpressPlusTM-PAGE gels (GenScript, USA).

Polyacrylamide Gel Electrophoresis:

Article Title: Sandwich‐Structured Implants to Obstruct Multipath Energy Supply and Trigger Self‐Enhanced Hypoxia‐Initiated Chemotherapy Against Postsurgical Tumor Recurrence and Metastasis
Article Snippet: .. Before sample loading, the samples used for reducing PAGE were mixed with a 5 × dithiothreitol‐contained buffer solution (Beyotime) at a 1 : 4 v/v ratio and denatured through heating (95 °C) and oscillation (1200 rpm) for 30 min in a shaking incubator (MSC‐100, Thermo, USA), and the samples used for nonreducing PAGE were thoroughly mixed with a 5 × loading buffer solution without any reductants or denaturants (Beyotime) through a vortex for 3 min at room temperature. .. Subsequently, the reduced and nonreduced samples were separately loaded onto a prefabricated SDS‐free polyacrylamide gel composed of 4% stacking gel and 20% separating gel (Beyotime).